Stemline demonstrates superior expansion of bone marrow hematopoietic stem cells (HSC). To test the ability of Stemline II Hematopoietic Stem Cell Expansion Medium to expand CD34+ HSCs, researchers at Sigma Aldrich and the University of Kentucky designed a bench-scale expansion assay. Cells were seeded into the wells of 24-well tissue culture plates. One milliliter of medium was added to each well with the appropriate cytokines to stimulate growth (100 ng/ml each of TPO, SCF, and G-CSF). Each condition was performed in triplicate and seeded with 10,000 cells per ml in each well. The cells were counted on day 14 and the fold increase was determined by cellsfinal/cellsinitial. HSCs from bone marrow cultured in Stemline and Stemline II demonstrated superior expansion to those grown in other serum-free HSC media.